Identification and analysis of gene families from the duplicated genome of soybean using EST sequences.

ABSTRACT:

Background

Large scale gene analysis of most organisms is hampered by incomplete genomic sequences. In many organisms, such as soybean, the best source of sequence information is the existence of expressed sequence tag (EST) libraries. Soybean has a large (1115 Mbp) genome that has yet to be fully sequenced. However it does have the 6th largest EST collection comprised of ESTs from a variety of soybean genotypes. Many EST libraries were constructed from RNA extracted from various genetic backgrounds, thus gene identification from these sources is complicated by the existence of both gene and allele sequence differences. We used the ESTminer suite of programs to identify potential soybean gene transcripts from a single genetic background allowing us to observe functional classificat

SUBMITTER: Nelson RT 

PROVIDER: S-EPMC1557498 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature