Circulating proviral HIV DNA and HIV-associated dementia.

ABSTRACT:

Objective

Individuals continue to develop HIV-1-associated dementia (HAD) despite treatment with highly active antiretroviral therapy (HAART). Monocytes/macrophages (M/MPhi) can harbor proviral DNA that is not eradicated by HAART. To determine if HAD is associated with the level of HIV-1 infection within circulating leukocytes, we quantified HIV-1 DNA copy number in peripheral blood mononuclear cells (PBMC), and in PBMC subsets.

Design

Cross-sectional analysis within the Hawaii Aging with HIV Cohort comparing participants with HAD to those with normal cognition (NC).

Methods

Real-time PCR assays assessing HIV DNA copy number/1 x 10 cells were performed on PBMC and subsets.

Results

Individuals with HAD (n = 27) had a median (interquartile range) of 9.11 (37.20)

SUBMITTER: Shiramizu B 

PROVIDER: S-EPMC1557628 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature