The effect of the stromal component of breast tumours on prediction of clinical outcome using gene expression microarray analysis.

ABSTRACT:

Introduction

The aim of this study was to examine the effect of the cellular composition of biopsies on the error rates of multigene predictors of response of breast tumours to neoadjuvant adriamycin and cyclophosphamide (AC) chemotherapy.

Materials and methods

Core biopsies were taken from primary breast tumours of 43 patients prior to AC, and subsequent clinical response was recorded. Post-chemotherapy (day 21) samples were available for 16 of these samples. Frozen sections of each core were used to estimate the proportion of invasive cancer and other tissue components at three levels. Transcriptional profiling was performed using a cDNA array containing 4,600 elements.

Results

Twenty-three (53%) patients demonstrated a 'good' and 20 (47%) a 'poor' clinical response

SUBMITTER: Cleator SJ 

PROVIDER: S-EPMC1557729 | biostudies-literature | 2006

REPOSITORIES: biostudies-literature