Inferring direct regulatory targets from expression and genome location analyses: a comparison of transcription factor deletion and overexpression.

ABSTRACT:

Background

Effects on gene expression due to environmental or genetic changes can be easily measured using microarrays. However, indirect effects on expression can be substantial. The indirect effects of a perturbation need to be distinguished from the direct effects if we are to understand the structure and behavior of regulatory networks.

Results

The most direct way to perturb a transcriptional network is to alter transcription factor activity. Here, for the first time, we compare expression changes and genomic binding in a simple regulon under conditions of both low and high transcription factor activity. Specifically, we assessed the effects on expression and binding due to deletion of the yeast LEU3 transcription factor gene and effects due to elevation of Leu3 activity

SUBMITTER: Tang L 

PROVIDER: S-EPMC1559704 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature