Differential DNA methylation profiles in gynecological cancers and correlation with clinico-pathological data.

ABSTRACT:

Background

Epigenetic gene silencing is one of the major causes of carcinogenesis. Its widespread occurrence in cancer genome could inactivate many cellular pathways including DNA repair, cell cycle control, apoptosis, cell adherence, and detoxification. The abnormal promoter methylation might be a potential molecular marker for cancer management.

Methods

For rapid identification of potential targets for aberrant methylation in gynecological cancers, methylation status of the CpG islands of 34 genes was determined using pooled DNA approach and methylation-specific PCR. Pooled DNA mixture from each cancer type (50 cervical cancers, 50 endometrial cancers and 50 ovarian cancers) was made to form three test samples. The corresponding normal DNA from the patients of each cancer

SUBMITTER: Yang HJ 

PROVIDER: S-EPMC1560388 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature