Genome-wide hierarchy of replication origin usage in Saccharomyces cerevisiae.

ABSTRACT: Replication origins in a genome are inherently different in their base sequence and in their response to temporal and cell cycle regulation signals for DNA replication. To investigate the chromosomal determinants that influence the efficiency of initiation of DNA replication genome-wide, we made use of a reverse strategy originally used for the isolation of replication initiation mutants in Saccharomyces cerevisiae. In yeast, replication origins isolated from chromosomes support the autonomous replication of plasmids. These replication origins, whether in the context of a chromosome or a plasmid, will initiate efficiently in wild-type cells but show a dramatically contrasted efficiency of activation in mutants defective in the early steps of replication initiation. Serial passages of a gen

SUBMITTER: Donato JJ 

PROVIDER: S-EPMC1560401 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature