Trans activity of the norovirus Camberwell proteinase and cleavage of the N-terminal protein encoded by ORF1.

ABSTRACT: The virus-encoded proteinase of Camberwell virus, a genogroup 2 norovirus, was synthesized in Escherichia coli. The purified proteinase had correct N and C termini and showed trans activity in cell-free assays. trans activity was also demonstrated in COS cells transfected with constructs encoding either the proteinase or a proteinase-polymerase fusion. The N-terminal protein of ORF1 was cleaved in COS cells, possibly at the site E(194)/S.

SUBMITTER: Seah EL 

PROVIDER: S-EPMC156193 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature