Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.

ABSTRACT: Genes involved in magnetite biomineralization are clustered within the genomic magnetosome island of Magnetospirillum gryphiswaldense. Their transcriptional organization and regulation were studied by several approaches. Cotranscription of genes within the mamAB, mamDC, and mms clusters was demonstrated by reverse transcription-PCR (RT-PCR) of intergenic regions, indicating the presence of long polycistronic transcripts extending over more than 16 kb. The transcription start points of the mamAB, mamDC, and mms operons were mapped at 22 bp, 52 bp, and 58 bp upstream of the first genes of the operons, respectively. Identified -10 and -35 boxes of the P(mamAB), P(mamDC), and P(mms) promoters showed high similarity to the canonical sigma(70) recognition sequence. The transcription of magnetoso

SUBMITTER: Schubbe S 

PROVIDER: S-EPMC1563640 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature