A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.

ABSTRACT: We present a new design for a fluorescence microspectrophotometer for use in kinetic crystallography in combination with x-ray diffraction experiments. The FLUMIX device (Fluorescence spectroscopy to monitor intermediates in x-ray crystallography) is built for 0 degrees fluorescence detection, which has several advantages in comparison to a conventional fluorometer with 90 degrees design. Due to the reduced spatial requirements and the need for only one objective, the system is highly versatile, easy to handle, and can be used for many different applications. In combination with a conventional stereomicroscope, fluorescence measurements or reaction initiation can be performed directly in a hanging drop crystallization setup. The FLUMIX device can be combined with most x-ray sources, normal

SUBMITTER: Klink BU 

PROVIDER: S-EPMC1563776 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature