All three variable regions of the TRIM5alpha B30.2 domain can contribute to the specificity of retrovirus restriction.

ABSTRACT: Recent studies have revealed the contribution of TRIM5alpha to retrovirus restriction in cells from a variety of primate species. TRIM5alpha consists of a tripartite motif (the RBCC domain) followed by a B30.2 domain. The B30.2 domain is thought to be involved in determination of restriction specificity and contains three variable regions. To investigate the relationship between the phylogeny of primate TRIM5alpha and retrovirus restriction specificity, a series of chimeric TRIM5alpha consisting of the human RBCC domain followed by the B30.2 domain from various primates was constructed. These constructs showed restriction profiles largely consistent with the origin of the B30.2 domain. Restriction specificity was further investigated with a variety of TRIM5alphas containing mixed or mutate

SUBMITTER: Ohkura S 

PROVIDER: S-EPMC1563890 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature