Hotspots of transcription factor colocalization in the genome of Drosophila melanogaster.

ABSTRACT: Regulation of gene expression is a highly complex process that requires the concerted action of many proteins, including sequence-specific transcription factors, cofactors, and chromatin proteins. In higher eukaryotes, the interplay between these proteins and their interactions with the genome still is poorly understood. We systematically mapped the in vivo binding sites of seven transcription factors with diverse physiological functions, five cofactors, and two heterochromatin proteins at approximately 1-kb resolution in a 2.9 Mb region of the Drosophila melanogaster genome. Surprisingly, all tested transcription factors and cofactors show strongly overlapping localization patterns, and the genome contains many "hotspots" that are targeted by all of these proteins. Several control experim

SUBMITTER: Moorman C 

PROVIDER: S-EPMC1567692 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature