The active FMR1 promoter is associated with a large domain of altered chromatin conformation with embedded local histone modifications.

ABSTRACT: We have analyzed the effects of gene activation on chromatin conformation throughout an approximately 170-kb region comprising the human fragile X locus, which includes a single expressed gene, FMR1 (fragile X mental retardation 1). We have applied three approaches: (i) chromosome conformation capture, which assesses relative interaction frequencies of chromatin segments; (ii) an extension of this approach that identifies domains whose conformation differs from the average, which we developed and named chromosome conformation profiling; and (iii) ChIP analysis of histone modifications. We find that, in normal cells where FMR1 is active, the FMR1 promoter is at the center of a large ( approximately 50 kb) domain of reduced intersegment interactions. In contrast, in fragile X cells where FMR

SUBMITTER: Gheldof N 

PROVIDER: S-EPMC1567902 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature