Identification of differentially expressed genes in cutaneous squamous cell carcinoma by microarray expression profiling.

ABSTRACT:

Background

Carcinogenesis is a multi-step process indicated by several genes up- or down-regulated during tumor progression. This study examined and identified differentially expressed genes in cutaneous squamous cell carcinoma (SCC).

Results

Three different biopsies of 5 immunosuppressed organ-transplanted recipients each normal skin (all were pooled), actinic keratosis (AK) (two were pooled), and invasive SCC and additionally 5 normal skin tissues from immunocompetent patients were analyzed. Thus, total RNA of 15 specimens were used for hybridization with Affymetrix HG-U133A microarray technology containing 22,283 genes. Data analyses were performed by prediction analysis of microarrays using nearest shrunken centroids with the threshold 3.5 and ANOVA analysis was independ

SUBMITTER: Nindl I 

PROVIDER: S-EPMC1569867 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature