Sodium (2-sulfonatoethyl) methanethiosulfonate prevents S-nitroso-L-cysteine activation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeca.

ABSTRACT: 1. The modulation of large conductance Ca(2+)-activated K(+) (BK(Ca)) channels by the nitric oxide (NO(.)) donor, S-nitroso-L-cysteine (NOCys) and three sulfhydryl-specific methanethiosulfonate (MTS) reagents, positively charged 2-aminoethyl MTS hydrobromide (MTSEA C(3)H(9)NO(2)S(2)HBr) and [2-(trimethylammonium) ethyl MTS bromide (MTSET C(6)H(16)NO(2)S(2)Br), and negatively charged sodium (2-sulfonatoethyl) MTS (MTSES C(3)H(7)O(5)S(3)Na) were compared in excised inside-out membrane patches of the guinea-pig taenia caeca. 2. In membrane patches bathed in a low Ca(2+) (15 nM) high K(+) physiological salt solution, 1-3 BK(Ca) channels opened with a low probability (N.P(o)) of 0.019+/-0.011 at 0 mV. N.P(o) readily increased with membrane depolarization, raised Ca(2+) concentration or upon the

SUBMITTER: Lang RJ 

PROVIDER: S-EPMC1573943 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature