Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep.

ABSTRACT:

Background

The sheep is an important model animal for testing novel fracture treatments and other medical applications. Despite these medical uses and the well known economic and cultural importance of the sheep, relatively little research has been performed into sheep genetics, and DNA sequences are available for only a small number of sheep genes.

Results

In this work we have sequenced over 47 thousand expressed sequence tags (ESTs) from libraries developed from healing bone in a sheep model of fracture healing. These ESTs were clustered with the previously available 10 thousand sheep ESTs to a total of 19087 contigs with an average length of 603 nucleotides. We used the newly identified sequences to develop RT-PCR assays for 78 sheep genes and measured differential expres

SUBMITTER: Hecht J 

PROVIDER: S-EPMC1578570 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature