Analysis of pleiotropic transcriptional profiles: a case study of DNA gyrase inhibition.

ABSTRACT: Genetic and environmental perturbations often result in complex transcriptional responses involving multiple genes and regulons. In order to understand the nature of a response, one has to account for the contribution of the downstream effects to the formation of a response. Such analysis can be carried out within a statistical framework in which the individual effects are independently collected and then combined within a linear model. Here, we modeled the contribution of DNA replication, supercoiling, and repair to the transcriptional response of inhibition of the Escherichia coli gyrase. By representing the gyrase inhibition as a true pleiotropic phenomenon, we were able to demonstrate that: (1) DNA replication is required for the formation of spatial transcriptional domains; (2) the tr

SUBMITTER: Jeong KS 

PROVIDER: S-EPMC1584274 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature