A powerful method for detecting differentially expressed genes from GeneChip arrays that does not require replicates.

ABSTRACT:

Background

Studies of differential expression that use Affymetrix GeneChip arrays are often carried out with a limited number of replicates. Reasons for this include financial considerations and limits on the available amount of RNA for sample preparation. In addition, failed hybridizations are not uncommon leading to a further reduction in the number of replicates available for analysis. Most existing methods for studying differential expression rely on the availability of replicates and the demand for alternative methods that require few or no replicates is high.

Results

We describe a statistical procedure for performing differential expression analysis without replicates. The procedure relies on a Bayesian integrated approach (BGX) to the analysis of Affymetrix GeneChips.

SUBMITTER: Hein AM 

PROVIDER: S-EPMC1586027 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature