Expression of steroidogenic factor 1 in the testis requires an interactive array of elements within its proximal promoter.

ABSTRACT: Steroidogenic factor 1 (SF-1) is an orphan nuclear receptor that is important for expression of genes involved in sexual differentiation, testicular and adrenal development, and hormone synthesis and regulation. To better understand the mechanisms required for SF-1 production, we employed transient transfection analysis and electrophoretic mobility shift assays to characterize the elements and proteins required for transcriptional activity of the SF-1 proximal promoter in testicular Sertoli and Leydig cells and adrenocortical cells. Direct comparison of SF-1-promoter activity in testis and adrenal cell types established that a similar set of regulatory elements (an E box, CCAAT box, and Sp1-binding sites) is required for proximal promoter activity in these cells. Further evaluation of the

SUBMITTER: Scherrer SP 

PROVIDER: S-EPMC1586108 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature