Development and validation of a bovine macrophage specific cDNA microarray.

ABSTRACT:

Background

The response of macrophages to danger signals is an important early stage in the immune response. Our understanding of this complex event has been furthered by microarray analysis, which allows the simultaneous investigation of the expression of large numbers of genes. However, the microarray resources available to study these events in livestock animals are limited.

Results

Here we report the development of a bovine macrophage specific (BoMP) cDNA microarray. The BoMP microarray contains 5026 sequence elements (printed in duplicate) and numerous controls. The majority of the clones incorporated on the microarray were derived from the BoMP cDNA library generated from bovine myeloid cells subjected to various stimuli, including over 900 sequences unique to the libr

SUBMITTER: Jensen K 

PROVIDER: S-EPMC1590031 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature