Analysis of the prostate cancer cell line LNCaP transcriptome using a sequencing-by-synthesis approach.

ABSTRACT:

Background

High throughput sequencing-by-synthesis is an emerging technology that allows the rapid production of millions of bases of data. Although the sequence reads are short, they can readily be used for re-sequencing. By re-sequencing the mRNA products of a cell, one may rapidly discover polymorphisms and splice variants particular to that cell.

Results

We present the utility of massively parallel sequencing by synthesis for profiling the transcriptome of a human prostate cancer cell-line, LNCaP, that has been treated with the synthetic androgen, R1881. Through the generation of approximately 20 megabases (MB) of EST data, we detect transcription from over 10,000 gene loci, 25 previously undescribed alternative splicing events involving known exons, and over 1,500 high

SUBMITTER: Bainbridge MN 

PROVIDER: S-EPMC1592491 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature