Matrix GLA protein function in human trabecular meshwork cells: inhibition of BMP2-induced calcification process.

ABSTRACT:

Purpose

The matrix GLA (MGP) gene has been found to be among the 10 most highly expressed genes in the human trabecular meshwork (TM), and its expression is affected by conditions associated with glaucoma. Because MGP protein has been shown to play a key role in inhibiting calcification in cartilage and arterial vessels, MGP's function in human TM was investigated.

Methods

Perfused TM tissue and primary human TM (HTM) cells originated from donors of nonglaucomatous eyes. MGP mRNA was assayed by relative quantitative and real-time PCR. AdhMGP recombinant adenovirus was generated by bacterial transposition. Western blot analyses were cross-reacted with MGP N-terminal- and conformational-specific antibodies. MGP/BMP2 colocalization was analyzed by confocal microscopy. gamma-Car

SUBMITTER: Xue W 

PROVIDER: S-EPMC1592516 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature