Multiplexed genetic analysis using an expanded genetic alphabet.

ABSTRACT:

Background

All states require some kind of testing for newborns, but the policies are far from standardized. In some states, newborn screening may include genetic tests for a wide range of targets, but the costs and complexities of the newer genetic tests inhibit expansion of newborn screening. We describe the development and technical evaluation of a multiplex platform that may foster increased newborn genetic screening.

Methods

MultiCode PLx involves three major steps: PCR, target-specific extension, and liquid chip decoding. Each step is performed in the same reaction vessel, and the test is completed in approximately 3 h. For site-specific labeling and room-temperature decoding, we use an additional base pair constructed from isoguanosine and isocytidine. We used the met

SUBMITTER: Johnson SC 

PROVIDER: S-EPMC1592527 | biostudies-literature | 2004 Nov

REPOSITORIES: biostudies-literature