Isw1 functions in parallel with the NuA4 and Swr1 complexes in stress-induced gene repression.

ABSTRACT: The packaging of DNA into chromatin allows eukaryotic cells to organize and compact their genomes but also creates an environment that is generally repressive to nuclear processes that depend upon DNA accessibility. There are several classes of enzymes that modulate the primary structure of chromatin to regulate various DNA-dependent processes. The biochemical activities of the yeast Isw1 ATP-dependent chromatin-remodeling enzyme have been well characterized in vitro, but little is known about how these activities are utilized in vivo. In this work, we sought to discern genetic backgrounds that require Isw1 activity for normal growth. We identified a three-way genetic interaction among Isw1, the NuA4 histone acetyltransferase complex, and the Swr1 histone replacement complex. Transcription

SUBMITTER: Lindstrom KC 

PROVIDER: S-EPMC1592817 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature