The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation.

ABSTRACT: Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in organisms as diverse as Saccharomyces cerevisiae, humans, and flies. For example, the well-studied yeast SAGA is related to three mammalian complexes. We previously identified Drosophila melanogaster orthologues of yeast SAGA components Ada2, Ada3, Spt3, and Tra1 and demonstrated that they associate with dGcn5 in a high-molecular-weight complex. To better understand the function of Drosophila SAGA (dSAGA), we sought to affinity purify and characterize this complex in more detail.

SUBMITTER: Guelman S 

PROVIDER: S-EPMC1592886 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature