Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body.

ABSTRACT: The spindle-pole body (SPB), the yeast analog of the centrosome, serves as the major microtubule (MT) organizing center in the yeast cell. In addition to this central function, the SPB organizes and concentrates proteins required for proper coordination between the nuclear-division cycle and cytokinesis. For example, the Schizosaccharomyces pombe septation-initiation network (SIN), which is responsible for initiating actomyosin ring constriction and septation, is assembled at the SPB through its two scaffolding components, Sid4 and Cdc11. In an effort to identify novel SIN interactors, we purified Cdc11 and identified by mass spectrometry a previously uncharacterized protein associated with it, Ppc89. Ppc89 localizes constitutively to the SPB and interacts directly with Sid4. A fusion betw

SUBMITTER: Rosenberg JA 

PROVIDER: S-EPMC1593159 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature