Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana.

ABSTRACT:

Background

Entomopathogenic fungi such as Beauveria bassiana are considered promising biological control agents for a variety of arthropod pests. Beauveria species, however, have the potential to elicit allergenic reactions in humans, although no specific allergens have been characterized to date.

Methods

Four putative allergens were identified within B. bassiana expressed sequence tag (EST) datasets. IgE-reactivity studies were performed using sera from patients displaying mold allergies against recombinant B. bassiana proteins expressed in E. coli.

Results

Full length cDNA and genomic nucleotide sequences of four potential B. bassiana allergens were isolated. BLASTX search results led to their putative designation as follows; Bb-Eno1, with similarity to fungal enola

SUBMITTER: Westwood GS 

PROVIDER: S-EPMC1594567 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature