Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.

ABSTRACT: In this study we report on the development and application of a novel method for efficiently extracting and detecting single Cryptosporidium oocysts from archived glass slides. Laser capture microscopy was used to extract low numbers of oocysts from archived glass slides. Highly sensitive real-time PCR methods were then developed to enable the rapid detection and identification of Cryptosporidium oocysts from these samples. The method was applied to fecal smears stained with a variety of standard oocyst stains and water samples. This application, with samples derived from both public health and water service laboratories, highlighted the strong potential of this method to be used as a rapid high-throughput screening tool for the routine monitoring of Cryptosporidium and other medically imp

SUBMITTER: Sunnotel O 

PROVIDER: S-EPMC1594691 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature