Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.
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ABSTRACT:
SUBMITTER: Mueller M
PROVIDER: S-EPMC1594769 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
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Identification of Borrelia burgdorferi ribosomal protein L25 by the phage surface display method and evaluation of the protein's value for serodiagnosis.
Mueller Markus M Bunk Sebastian S Diterich Isabel I Weichel Michael M Rauter Carolin C Hassler Dieter D Hermann Corinna C Crameri Reto R Hartung Thomas T
Journal of clinical microbiology 20061001 10
The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined w ...[more]
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