Genotyping of measles virus in clinical specimens on the basis of oligonucleotide microarray hybridization patterns.

ABSTRACT: An oligonucleotide microarray hybridization method for identification of most known measles virus (MV) genotypes was developed. Like the conventional genotyping method, the microarray relied on detecting sequence differences in the 450-nucleotide region coding for the COOH-terminal 150 amino acids of the nucleoprotein (N). This region was amplified using PCR primers binding to all known MV genotypes. The microarray included 71 pairs of oligonucleotide probes (oligoprobes) immobilized on glass slides. Each pair consisted of a genotype-specific oligoprobe, which matched the sequence of only one target genotype, and a control oligoprobe, which contained mismatches at the nucleotide positions unique to this genotype. A pattern recognition algorithm based on cluster analysis of the ratios of hy

SUBMITTER: Neverov AA 

PROVIDER: S-EPMC1594792 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature