Development of an enzyme immunoassay for detection of sapovirus-specific antibodies and its application in a study of seroprevalence in children.

ABSTRACT: Sapoviruses (SVs) are an important cause of acute pediatric gastroenteritis. Due to the lack of appropriate diagnostic methods, the epidemiology of SV-associated illness remains poorly understood. Baculovirus and Escherichia coli expression systems were evaluated for the development of antibody detection enzyme immunoassays (EIA). Age-related antibody prevalence in children was studied using the new EIA. Because of the low yield of the baculovirus system, the E. coli-expressed SV capsid proteins were used to develop the EIA. The antigenic specificities of the E. coli-expressed SV capsid proteins were demonstrated using hyperimmune antisera raised in animals and sera collected from patients. A high prevalence (>90%) of antibodies to both SV (strain Mex340) and norovirus (strain VA387) was o

SUBMITTER: Farkas T 

PROVIDER: S-EPMC1594811 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature