Comparative genomic hybridization analysis of Enterococcus faecalis: identification of genes absent from food strains.

ABSTRACT: Enterococcus faecalis, a member of the natural microbiota of animal and human intestinal tracts, is also present as a natural contaminant in a variety of fermented foods. Over the last decade, E. faecalis has emerged as a major cause of nosocomial infections. We investigated the genetic diversity in 30 clinical and food isolates, including strains V583 and MMH594, in order to determine whether clinical and food isolates could be distinguished. Data were obtained using comparative genomic hybridization and specific PCR with a total of 202 probes of E. faecalis, selected using the available V583 genome sequence and part of the MMH594 pathogenicity island. The cognate genes encoded mainly exported proteins. Hybridization data were analyzed by a two-component mixture model that estimates the p

SUBMITTER: Lepage E 

PROVIDER: S-EPMC1595521 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature