Autophagy-mediated reentry of Francisella tularensis into the endocytic compartment after cytoplasmic replication.

ABSTRACT: Intracellular bacterial pathogens evade the bactericidal functions of mammalian cells by physical escape from their phagosome and replication into the cytoplasm or through the modulation of phagosome maturation and biogenesis of a membrane-bound replicative organelle. Here, we detail in murine primary macrophages the intracellular life cycle of Francisella tularensis, a highly infectious bacterium that survives and replicates within mammalian cells. After transient interactions with the endocytic pathway, bacteria escaped from their phagosome by 1 h after infection and underwent replication in the cytoplasm from 4 to 20 h after infection. Unexpectedly, the majority of bacteria were subsequently found to be enclosed within large, juxtanuclear, LAMP-1-positive vacuoles called Francisella-con

SUBMITTER: Checroun C 

PROVIDER: S-EPMC1600002 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature