Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction.

ABSTRACT: Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

SUBMITTER: Luce AC 

PROVIDER: S-EPMC1602074 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature