Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies.

ABSTRACT: Isolation and self-replication of infectious HCV has been a difficult task. However, this is needed for the purposes of developing rational drugs and for the analysis of the natural virus. Our recent report of an in vitro system for the isolation of human HCV from infected patients and their replication in tissue culture addresses this challenge. At California Institute of Molecular Medicine several isolates of HCV, called CIMM-HCV, were grown for over three years in cell culture. This is a report of the analysis of CIMM-HCV isolates for subtypes and quasispecies using a 269 bp segment of the 5'UTR. HCV RNA from three patients and eleven CIMM-HCV were analyzed for this purpose. All isolates were essentially identical. Isolates of HCV from one patient were serially transmitted into fresh ce

SUBMITTER: Revie D 

PROVIDER: S-EPMC1609102 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature