Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases.

ABSTRACT:

Background

Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated through homologous recombination between the HSV genome and a recombination plasmid, which usually requires laborious screening or selection and can take several months. Recent advances in bacterial artificial chromosome (BAC) technology have enabled cloning of the whole HSV genome as a BAC plasmid and subsequent manipulation in E. coli. Thus, we sought a method to generate recombinant oncolytic HSV vectors more easily and quickly using BAC technology.

Results

We have developed an HSV-BAC system, termed the Flip-Flop HSV-BAC system, for the rapid generation o

SUBMITTER: Kuroda T 

PROVIDER: S-EPMC1609115 | biostudies-literature | 2006 Sep

REPOSITORIES: biostudies-literature