New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis.

ABSTRACT:

Background

Johne's disease, a serious chronic form of enteritis in ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). As the organism is very slow-growing and fastidious, several PCR-based methods for detection have been developed, based mainly on the MAP-specific gene IS900. However, because this gene is similar to genes in other mycobacteria, there is a need for sensitive and reliable methods to confirm the presence of MAP. As described here, two new real-time PCR systems on the IS900 gene and one on the F57 gene were developed and carefully validated on 267 strains and 56 positive clinical faecal samples.

Results

Our confirmatory PCR systems on IS900 were found sensitive and specific, only yielding weak false positive reactions in one strain for ea

SUBMITTER: Herthnek D 

PROVIDER: S-EPMC1609169 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature