Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR.

ABSTRACT:

Background

Control genes, which are often referred to as housekeeping genes, are frequently used to normalise mRNA levels between different samples. However, the expression level of these genes may vary among tissues or cells and may change under certain circumstances. Thus, the selection of housekeeping genes is critical for gene expression studies. To address this issue, 7 candidate housekeeping genes including several commonly used ones were investigated in isolated human reticulocytes. For this, a simple DeltaCt approach was employed by comparing relative expression of 'pairs of genes' within each sample. On this basis, stability of the candidate housekeeping genes was ranked according to repeatability of the gene expression differences among 31 samples.

Results

Initial

SUBMITTER: Silver N 

PROVIDER: S-EPMC1609175 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature