An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding.

ABSTRACT: Molecular surveys of meiofaunal diversity face some interesting methodological challenges when it comes to interstitial nematodes from soils and sediments. Morphology-based surveys are greatly limited in processing speed, while barcoding approaches for nematodes are hampered by difficulties of matching sequence data with traditional taxonomy. Intermediate technology is needed to bridge the gap between both approaches. An example of such technology is video capture and editing microscopy, which consists of the recording of taxonomically informative multifocal series of microscopy images as digital video clips. The integration of multifocal imaging with sequence analysis of the D2D3 region of large subunit (LSU) rDNA is illustrated here in the context of a combined morphological and barcode

SUBMITTER: De Ley P 

PROVIDER: S-EPMC1609217 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature