Culture-independent analysis of indomethacin-induced alterations in the rat gastrointestinal microbiota.

ABSTRACT: Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of approximately 1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quanti

SUBMITTER: Dalby AB 

PROVIDER: S-EPMC1610281 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature