A gene expressed during sexual and asexual sporulation in Phytophthora infestans is a member of the Puf family of translational regulators.

ABSTRACT: A gene from Phytophthora infestans that was previously identified as being induced during the development of sexual spores was also found to be active during asexual sporulation. The gene, M90, was expressed as a 3.1-kb primary transcript containing two introns and was predicted to encode a member of the Puf family of translational regulators. The protein showed up to 51% amino acid identity to other Puf proteins within its 353-amino-acid RNA-binding domain. Little similarity extended beyond this region, as noted for other members of the family. Expression of M90 was measured by using RNA blots and transformants of P. infestans expressing a fusion between the M90 promoter and the beta-glucuronidase (GUS) gene. A 1.3-kb promoter fragment conferred the normal M90 pattern of expression to the

SUBMITTER: Cvitanich C 

PROVIDER: S-EPMC161445 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature