Repression of phenazine antibiotic production in Pseudomonas aureofaciens strain 30-84 by RpeA.

ABSTRACT: Pseudomonas aureofaciens strain 30-84 is a biological control bacterium that utilizes a two-component GacS/GacA regulatory system interconnected with the PhzR/PhzI quorum sensing system to positively regulate biosynthesis of phenazine antibiotics that contribute to its association with plant hosts. To date, no negative regulators of phenazine production have been identified, nor has the role of repression been studied. Here we describe a novel repressor of secondary metabolism in P. aureofaciens strain 30-84, RpeA, whose deduced amino acid sequence is similar to those of a group of putative two-component regulatory systems of unknown function found in several animal and plant-pathogenic bacteria. In minimal medium where phenazine production is very low, inactivation of the rpeA gene enhanc

SUBMITTER: Whistler CA 

PROVIDER: S-EPMC161564 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature