Anti-human Olig2 antibody as a useful immunohistochemical marker of normal oligodendrocytes and gliomas.

ABSTRACT: Olig2 is a recently identified transcription factor involved in the phenotype definition of cells in the oligodendroglial lineage. The expression of Olig2 transcript has been demonstrated in human oligodendroglial tumors, although the protein expression has not been studied extensively. We developed a polyclonal antibody to human Olig2 and analyzed it immunohistochemically. The antibody depicted a single distinct band of predicted molecular weight by Western blotting, and did not cross-react with human Olig1. In normal human brain tissue, the nuclei of oligodendrocytes of interfascicular, perivascular, and perineuronal disposition were clearly labeled by the antibody. Similarly, the nuclei of oligodendroglial tumors were labeled. There was no apparent correlation between the staining inten

SUBMITTER: Yokoo H 

PROVIDER: S-EPMC1615653 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature