Comparison of nanogel drug carriers and their formulations with nucleoside 5'-triphosphates.

ABSTRACT:

Purpose

The aim of the study is to synthesize and characterize nanogel carriers composed of amphiphilic polymers and cationic polyethylenimine for encapsulation and delivery of cytotoxic nucleoside analogs 5'-triphosphates (NTPs) into cancer cells.

Methods

Nanogels were synthesized by a novel micellar approach and compared with carriers prepared by the emulsification/evaporation method. Complexes of nanogels with NTP were prepared; particle size and in vitro drug release were characterized. Resistance of the nanogel-encapsulated NTP to enzymatic hydrolysis was analyzed by ion-pair high-performance liquid chromatography. Binding to isolated cellular membranes, cellular accumulation and cytotoxicity were compared using breast carcinoma cell lines CL-66, MCF-7, and MDA-MB-231.

SUBMITTER: Vinogradov SV 

PROVIDER: S-EPMC1615883 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature