Identification of signature and primers specific to genus Pseudomonas using mismatched patterns of 16S rDNA sequences.

ABSTRACT:

Background

Pseudomonas, a soil bacterium, has been observed as a dominant genus that survives in different habitats with wide hostile conditions. We had a basic assumption that the species level variation in 16S rDNA sequences of a bacterial genus is mainly due to substitutions rather than insertion or deletion of bases. Keeping this in view, the aim was to identify a region of 16S rDNA sequence and within that focus on substitution prone stretches indicating species level variation and to derive patterns from these stretches that are specific to the genus.

Results

Repeating elements that are highly conserved across different species of Pseudomonas were considered as guiding markers to locate a region within the 16S gene. Four repeating patterns showing more than 80% consist

SUBMITTER: Purohit HJ 

PROVIDER: S-EPMC161812 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature