Infectivity and replication capacity of drug-resistant human immunodeficiency virus type 1 variants isolated during primary infection.

ABSTRACT: It is believed that replication capacity is an important determinant of human immunodeficiency virus type 1 (HIV-1) pathogenicity and transmissibility. To explore this, we conducted a comprehensive analysis of the replication properties of nine drug-resistant and nine drug-susceptible viral isolates derived from patients with primary HIV-1 infection. Viral isolates were tested for single-cycle infectivity in the GHOST cell line. The infectivity of isolates carrying resistance-associated mutations was significantly higher than that of drug-susceptible isolates. Additionally, the growth kinetics of these isolates were determined in CD4+ T lymphocytes. Drug-resistant isolates replicated as well as drug-susceptible viruses. Insertion of the resistance-conferring regions into an NL4-3-based mol

SUBMITTER: Simon V 

PROVIDER: S-EPMC161921 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature