Selection of small-colony variants of Salmonella enterica serovar typhimurium in nonphagocytic eucaryotic cells.

ABSTRACT: Salmonella enterica strains are enteropathogenic bacteria that survive and proliferate within vacuolar compartments of epithelial and phagocytic cells. Recently, it has been reported that fibroblast cells are capable of restricting S. enterica serovar Typhimurium intracellular growth. Here, we show that prolonged residence of bacteria in the intracellular environment of fibroblasts results in the appearance of genetically stable small-colony variants (SCV). A total of 103 SCV isolates, obtained from four independent infections, were subjected to phenotypic analysis. The following phenotypes were observed: (i) delta-aminolevulinic acid auxotrophy; (ii) requirement for acetate or succinate for growth in glucose minimal medium; (iii) auxotrophy for aromatic amino acids; and (iv) reduced growt

SUBMITTER: Cano DA 

PROVIDER: S-EPMC161971 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature