TILLING is an effective reverse genetics technique for Caenorhabditis elegans.

ABSTRACT:

Background

TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetic technique based on the use of a mismatch-specific enzyme that identifies mutations in a target gene through heteroduplex analysis. We tested this technique in Caenorhabditis elegans, a model organism in which genomics tools have been well developed, but limitations in reverse genetics have restricted the number of heritable mutations that have been identified.

Results

To determine whether TILLING represents an effective reverse genetic strategy for C. elegans we generated an EMS-mutagenised population of approximately 1500 individuals and screened for mutations in 10 genes. A total of 71 mutations were identified by TILLING, providing multiple mutant alleles for every gene tested. Some of th

SUBMITTER: Gilchrist EJ 

PROVIDER: S-EPMC1626091 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature