Ab initio modeling of the herpesvirus VP26 core domain assessed by CryoEM density.

ABSTRACT: Efforts in structural biology have targeted the systematic determination of all protein structures through experimental determination or modeling. In recent years, 3-D electron cryomicroscopy (cryoEM) has assumed an increasingly important role in determining the structures of these large macromolecular assemblies to intermediate resolutions (6-10 A). While these structures provide a snapshot of the assembly and its components in well-defined functional states, the resolution limits the ability to build accurate structural models. In contrast, sequence-based modeling techniques are capable of producing relatively robust structural models for isolated proteins or domains. In this work, we developed and applied a hybrid modeling approach, utilizing cryoEM density and ab initio modeling to pro

SUBMITTER: Baker ML 

PROVIDER: S-EPMC1626159 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature