Identification of novel regulators in T-cell differentiation of aplastic anemia patients.

ABSTRACT:

Background

Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular immune response. To overcome this technical problem we performed a microarray analysis of CD3+ T-cells derived from bone marrow aspirates and peripheral blood samples of newly diagnosed AA patients and healthy volunteers. Two AA patients were additionally analyzed after achieving a partial remission following immunosuppression. The regulation of selected candidate genes was confirmed by real-time RT-PCR.

Results

Among more than 22,200 transcripts, 583 genes were differentially expressed in the bone marrow of AA patients compared to health

SUBMITTER: Franzke A 

PROVIDER: S-EPMC1626471 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature