Replication of a hepatitis C virus replicon clone in mouse cells.

ABSTRACT:

Background

Hepatitis C Virus (HCV) is a significant public health burden and small animal models are needed to study the pathology and immunobiology of the virus. In effort to develop experimental HCV mouse models, we screened a panel of HCV replicons to identify clones capable of replicating in mouse hepatocytes.

Results

We report the establishment of stable HCV replication in mouse hepatocyte and fibroblast cell lines using replicons derived from the JFH-1 genotype 2a consensus sequence. Viral RNA replication efficiency in mouse cells was comparable to that observed in human Huh-7 replicon cells, with negative-strand HCV RNA and the viral NS5A protein being readily detected by Northern and Western Blot analysis, respectively. Although HCV replication was established in the

SUBMITTER: Uprichard SL 

PROVIDER: S-EPMC1635043 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature